Chloroquine diphosphate CAS NO.50-63-5

1,Best Quality:

Our products have exported to Germany, Spain, UK, USA, Australia, Middle East, and so on other countries, and we have got very good feedback from our customers.so you can trust us.

2, Payment method: for example T/T, Westerm Union,other

3, Excellent Service: Best service and after-sales service to all clients.

   1)We will ship the goods within 5days after get your payments.If you want to cancel or change order, please tell me within 24hours after you finish the payment..

   2)We will ship the goods that you order from us by DHL, UPS, TNT and EUB.We will decided to choose which courier depend on different countries.To find the best way to delivery the goods for you.

   3)As usual you can get the goods with in 4-7days.If the goods were lost or not received for other reasons, please contact us immediately.

4,Fast and safe delivery:

Sample Order : As usual you can get the goods within 4-7days. We can send it via HKems, HK Air Post, DHL or other methods.

We have a professional and stable logistics, and we can deliver the package smoothly around 3 to 5 days.

Bulk Order: Shipped via sea or air, it's according to your requirement, delivery time is 7-15days after payment.

If you have any other questions to consult me,please not hesitate to contact me any times.

In vitro studies Chloroquine (CHQ, 20 μM) inhibits IL-12p70 release and reduces Th1 priming ability of activated human monocyte-derived Langerhans-like cells (MoLC). Chloroquine (CHQ, 20 μM) enhanced IL-1 induced IL-23 secretion in MoLC, and subsequently increased IL-17A release by evoked CD4 + T cells. Chloroquine (25 μM) inhibited the expression of MMP-9 mRNA in normoxic and hypoxia in parental MDA-MB-231 cells. Chloroquine has cellular, dose, and hypoxia-dependent effects on MMP-2, MMP-9, and MMP-13 mRNA expression. Inhibition of TLR7 and TLR9 with IRS-954 or chloroquine can significantly reduce the proliferation of HuH7 cells in vitro.

In vivo studies Chloroquine (80 mg / kg, ip) did not prevent the growth of triple-negative MDA-MB-231 cells with high or low TLR9 expression levels in an orthotopic mouse model. TLR7 and TLR9 inhibition using IRS-954 or chloroquine significantly inhibited tumor growth in a mouse xenograft model. HCC development in the DEN / NMOR rat model was also significantly inhibited by chloroquine.

Cell experiments Cells were cultured in a 6-well plate with normal medium in the presence of a carrier or 25 or 50 μM chloroquine until near confluence, after which they were rinsed with sterile phosphate buffered saline (PBS) and further cultured for the indicated time. In serum-free medium. At the desired time point, the medium was discarded and the cells were quickly harvested in lysis buffer and clarified by centrifugation. After boiling the supernatant in a reduced sodium dodecyl sulfate (SDS) sample buffer, load equal amounts of protein (100 μg) per lane, and electrophorese the samples onto a 10 or 4-20% gradient polyacrylamide SDS gel And then transferred to a nitrocellulose membrane. membrane. To detect TLR9, the blots were incubated with anti-TLR9 antibodies overnight at 4 ° C and diluted 1: 500 in Tris buffered saline containing 0.1% (v / v) Tween-20 (TBST). Equal loading was confirmed with polyclonal rabbit anti-actin. Secondary detection was performed with a horseradish peroxidase-linked secondary antibody. Visualize protein bands with chemiluminescence using ECL kit

 

The images only for reference.